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31.
Danny Bavli Elishai Ezra Daniel Kitsberg Margarita Vosk-Artzi Shashi K. Murthy Yaakov Nahmias 《Biomicrofluidics》2016,10(2)
Cell-cell interactions play a key role in regeneration, differentiation, and basic tissue function taking place under physiological shear forces. However, current solutions to mimic such interactions by micro-patterning cells within microfluidic devices have low resolution, high fabrication complexity, and are limited to one or two cell types. Here, we present a microfluidic platform capable of laminar patterning of any biotin-labeled peptide using streptavidin-based surface chemistry. The design permits the generation of arbitrary cell patterns from heterogeneous mixtures in microfluidic devices. We demonstrate the robust co-patterning of α-CD24, α-ASGPR-1, and α-Tie2 antibodies for rapid isolation and co-patterning of mixtures of hepatocytes and endothelial cells. In addition to one-step isolation and patterning, our design permits step-wise patterning of multiple cell types and empty spaces to create complex cellular geometries in vitro. In conclusion, we developed a microfluidic device that permits the generation of perfusable tissue-like patterns in microfluidic devices by directly injecting complex cell mixtures such as differentiated stem cells or tissue digests with minimal sample preparation. 相似文献
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ABSTRACTDue to budget constraints, schools in the United States have increasingly turned to community arts organizations for support. School-community arts partnership stakeholders collaborate because of shared missions to provide students with valuable arts learning experiences. Investigations of these initiatives indicate that these partnerships improve arts learning opportunities and increase public support and resources for arts education. However, not much is known about the experiences and perspectives of the arts organizations that participate in these partnerships. Coordinating collective efforts with a multitude of institutions and interests poses challenges. In this study, we examine survey data collected from arts organization administrators who participated in a large-scale school-community arts partnership initiative. We find that these organizations are generally positive about their impacts on students’ educational outcomes, but there is substantial variation in these views. We also find that organizations differ in their levels of support for these collective efforts. Sources of this variation appear to be attributable to organizations’ preexisting resources and extent to which they are established. While this difference in levels of support is potentially inevitable, we find evidence that the operations handled by the “backbone” organization, i.e. the initiative’s facilitators and overseers, can significantly influence organizations’ levels of support for these efforts. Organizations are more likely to support these collaborative efforts when they believe the backbone organization ensures transparency with initiative operations, provides regular, effective communication, and effectively resolves competing priorities. 相似文献
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Heather A. Starnes Meghan H. McDonough Jeffrey S. Wilson Daniel K. Mroczek Francine Laden Philip J. Troped 《Measurement in physical education and exercise science》2019,23(2):135-147
The purpose of this study was to examine the factorial invariance of the Abbreviated Neighborhood Environment Walkability Scale (NEWS-A) across subgroups based on demographic, health-related, behavioral, and environmental characteristics among Nurses’ Health Study participants (N = 2,919; age M = 73.0, SD = 6.9 years) living in California, Massachusetts, and Pennsylvania. A series of multigroup confirmatory factor analyses were conducted to evaluate increasingly restrictive hypotheses of factorial invariance. Factorial invariance was supported across age, walking limitations, and neighborhood walking. Only partial scalar invariance was supported across state residence and neighborhood population density. This evidence provides support for using the NEWS-A with older women of different ages, who have different degrees of walking limitations, and who engage in different amounts of neighborhood walking. Partial scalar invariance suggests that researchers should be cautious when using the NEWS-A to compare older adults living in different states and neighborhoods with different levels of population density. 相似文献
36.
Fostering College-Going Expectations of Immigrant Students Through the Sympathetic Touch of School Leadership 总被引:1,自引:0,他引:1
Daniel D. Liou 《Multicultural Perspectives》2016,18(2):82-90
This article intends to support the efforts of administrators, teachers, and community activists to center race, equity, and anti-deficit perspectives within the practice of school leadership. By drawing upon methods of critical race studies, and Du Bois's 1935 concept of the sympathetic touch, the author provides examples of anti-deficit approaches to sympathetic leadership in a large, diverse high school in California. Through reflections and recollections upon this case study, the author underscores ways for school leaders to formulate the conditions for college-going expectations for low-income immigrant students. This retrospective account includes concrete leadership examples, political strategies, and scenarios to illuminate the relevance and utilities of the sympathetic touch in educational leadership. In effect, the article calls for school leaders to harness anti-deficit and anti-oppressive mindsets in the work of inclusion, equity, and social justice. 相似文献
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Mauricio Garzon Olivier Dupuy Laurent Bosquet Anil Nigam Alain Steve Comtois Martin Juneau 《European Journal of Sport Science》2017,17(3):310-316
This study compared heart rate recovery (HRR) after incremental maximal exercise performed at the same external power output (Pext) on dry land ergocycle (DE) vs. immersible ergocycle (IE). Fifteen young healthy participants (30?±?7 years, 13 men and 2 women) performed incremental maximal exercise tests on DE and on IE. The initial Pext on DE was 25?W and was increased by 25?W/min at a pedalling cadence between 60 and 80?rpm, while during IE immersion at chest level in thermoneutral water (30°C), the initial Pext deployment was at a cadence of 40?rpm which was increased by 10?rpm until 70?rpm and thereafter by 5?rpm until exhaustion. Gas exchange and heart rate (HR) were measured continuously during exercise and recovery for 5?min. Maximal HR (DE: 176?±?15 vs. IE 169?±?12?bpm) reached by the subjects in the two conditions did not differ (P?>?.05). Parasympathetic reactivation parameters (ΔHR from 10 to 300?s) were compared during the DE and IE HR recovery recordings. During the IE recovery, parasympathetic reactivation in the early phase was more predominant (HRR at Δ10–Δ60?s, P?<?.05), but similar in the late phase (HRR at Δ120–Δ300?s, P?>?.05) when compared to the DE condition. In conclusion, incremental maximal IE exercise at chest level immersion in thermoneutral water accelerates the early phase parasympathetic reactivation compared to DE in healthy young participants. 相似文献
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Marc Torka 《Minerva》2018,56(1):59-83
Funding is an important mechanism for exercising influence over ever more parts of academic systems. In order to do so, funding agencies attempt to export their functional and normative prerequisites for financing to new fields. One essential requirement for fundees is then to construct research processes in the form of a project beforehand, one that is limited in time, scope and content. This article demonstrates how the public funding of doctoral programs expands this model of project research from experienced academics to the socialization process for the new academic generation. This process of “projectification” underlies funding-driven institutional changes in doctoral training. A multi-level comparative study of German policies, funding mechanisms and organizational frameworks for doctoral training demonstrates the emergence of a specific model of predefined PhD projects. The investigation of doctoral training practices reveals that socio-epistemic preconditions regulate whether research fields adopted or rejected this demanding model. This result contradicts widespread claims about a radical change in doctoral training and suggests focusing on the actual practices of field-specific doctoral research. 相似文献
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José Roberto Andrade do Nascimento-Junior Leonardo de Sousa Fortes Gabriel Lucas Morais Freire Daniel Vicentini de Oliveira Lenamar Fiorese Lorcan Donal Cronin 《Measurement in physical education and exercise science》2020,24(1):11-24
ABSTRACTThis research adapted the Life Skills Scale for Sport (LSSS) into Portuguese and provided evidence for its construct validity. Study 1 included four translators and five academics who developed a Portuguese version of the LSSS (P-LSSS). During this study, evidence for the content and substantive aspects of construct validity was provided using an expert panel and 25 sports participants. Study 2 included 413 participants that completed the P-LSSS. Within this study, evidence for the structural aspect of construct validity was provided via factor analyses. Study 3 included 134 participants who completed the P-LSSS and a measure of motivation. This study provided evidence for the external aspect of construct validity, with results showing more self-determined motivation had positive relationships with participant’s life skills development. Overall, our findings provided evidence for the construct validity of P-LSSS scores. Researchers and practitioners can use the P-LSSS to assess life skills development within sports participants. 相似文献